Remove All Borgia Flags in Rome.
Receive 100% synch in ACB and full synch in all missions of the Da Vinci Disappearance.
Beat up the 5 thirsty harlequins.
Solve all of Subject 16's puzzles.
Steal 5 horses from their riders, while remaining on horseback.
Double Assassinate from a parachute.
Kill 10 guards with a single arrow storm.
Throw a Long Weapon, Heavy Weapon, and Smoke bomb more than 10 m at a guard.
Win 10000 florins playing Hazard.
Win the highest bet at the Fights.
Complete the Da Vinci Disappearance.
Kill a guard with the bag that drops from a lift.
Pay 500 florins to an orator, then pickpocket him afterward.
Kill a guard with a broom.
Secure the Apple of Eden.
Complete DNA Sequence 9.
Complete DNA Sequence 8.
Jump with the parachute off the top of Castel Sant'Angelo.
Discover the Shrine in the Basilica di San Pietro.
Complete DNA Sequence 7.
Complete 10 Guild Challenges.
Discover the Shrine in the Cloaca Maxima.
Discover the Shrine in Palazzo Laterano.
Collect All Feathers.
Burn All Borgia Towers.
Complete DNA Sequence 6.
Destroy the Tank.
Complete DNA Sequence 5.
Destroy the Naval Cannon.
Earn 3 gold medals in the Animus Virtual Training Program.
Train a recruit up to the Rank of Assassin.
Complete DNA Sequence 4.
Destroy the Bomber.
Destroy the Machine Gun.
Recruit 3 Assassins.
Complete DNA Sequence 3.
Achieve 100% Synchronization in any Sequence other than Sequence 1.
Renovate 5 buildings in the Antico district.
Discover the Shrine in Il Colosseo.
Discover the Shrine in the Catacombe di Roma.
Complete DNA Sequence 2.
Discover the Shrine in the Terme di Traiano.
Perform an Execution Streak of 10 kills.
Replay a Memory.
Throw money in a well.
Find 1 Artifact in 2012.
Access your email in 2012.
Find a secure place to hide and re-enter the Animus.
Complete DNA Sequence 1.
Attempt to access DNA Sequence 9 for the first time.
Complete an entire Session with at least one kill (Multiplayer Only).
Kill your target while hidden in a hay bale (Multiplayer Only).
Take the lead 10 seconds before the end of a session and go on to win (Multiplayer Only).
Kill your target and escape your pursuer in less than 10 seconds (Multiplayer Only).
During Open Conflict, kill your target and escape (Multiplayer Only).
Reach Level 50 (Multiplayer Only).
Get all the Co-op bonuses in 1 session (Multiplayer Only).
Score 750 points or more on a single kill (Multiplayer Only).
Get every single bonus at least once (Multiplayer Only).
Perform a double or triple escape (Multiplayer Only).